BBC3 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

BCL2 binding component 3

2°C to 8°C|-20°C

NM_001127240.2

Bcl-2-binding component 3

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to p53 Upregulated Modulator Of Apoptosis (PUMA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to p53 Upregulated Modulator Of Apoptosis (PUMA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain p53 Upregulated Modulator Of Apoptosis (PUMA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of p53 Upregulated Modulator Of Apoptosis (PUMA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates, cell lysates and other biological fluids