REG3A ELISA Kit (Mouse)

ELISA Kit

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Reg3a

175

Bactericidal C-type lectin (By similarity). Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.

NP_035389.1

This assay has high sensitivity and excellent specificity for detection of Regenerating Islet Derived Protein 3 Alpha (REG3a).No significant cross-reactivity or interference between Regenerating Islet Derived Protein 3 Alpha (REG3a) and analogues was observed.

Component: Amount
Anti-REG3A Microplate: 96 Wells (12 x 8 Well strips)
REG3A Lyophilized Standard: 2
100X Biotinylated REG3A Detector Antibody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
Stop Solution: 1 x 6 mL
TMB Substrate: 1 x 9 mL

3h

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 3 Alpha (REG3a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 3 Alpha (REG3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 3 Alpha (REG3a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 3 Alpha (REG3a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%