Comparison

ADAM9 Chemi-Luminescent ELISA Kit (Rat)

Item no. OKCD03280
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Rat (Rattus norvegicus)
Sensitivity 0.156 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias a disintegrin and metallopeptidase domain 9 (meltrin gamma);a disintegrin and metalloproteinase domain 9 (meltrin gamma);disintegrin and metalloproteinase domain-containing protein 9;MDC9;meltrin gamma.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Adam9
Gene Fullname
ADAM metallopeptidase domain 9
Reconstitution and storage
2°C to 8°C|-20°C
Protein name
ADAM9
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Adam9 Microplate: 96 Wells (12 x 8 Well strips)
Adam9 Lyophilized Standard: 2
100X Adam9 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 9 (ADAM9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 9 (ADAM9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the A Disintegrin And Metalloprotease 9 (ADAM9) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 9 (ADAM9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 9 (ADAM9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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