AIFM1 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

apoptosis inducing factor mitochondria associated 1

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.

NP_001124318

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 85-101% - 91-98% - 90-101% - 93-101%
EDTA Plasma (n=5) - 91-99% - 82-101% - 78-98% - 91-102%
Heparin Plasma (n=5) - 89-97% - 90-104% - 88-97% - 85-101%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 79-102 - 81
EDTA Plasma (n=5) - 98-105 - 101
Heparin Plasma (n=5) - 78-95 - 81

Serum, plasma, tissue homogenates and other biological fluids