Comparison

CDCP1 Chemi-Luminescent ELISA Kit (Human)

Item no. OKCD03364
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity 0.312 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CD318;CUB domain-containing protein 1;membrane glycoprotein gp140;SIMA135;subtractive immunization M plus HEp3-associated 135 kDa protein;transmembrane and associated with src kinases;TRASK.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
CDCP1
Gene Fullname
CUB domain containing protein 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Nucleotide accession_num
NM_022842
Protein accession_num
NP_073753
Protein name
CUB domain-containing protein 1
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
CDCP1 Microplate: 96 Wells (12 x 8 Well strips)
CDCP1 Lyophilized Standard: 2
100X CDCP1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to CUB Domain Containing Protein 1 (CDCP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CUB Domain Containing Protein 1 (CDCP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the CUB Domain Containing Protein 1 (CDCP1) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CUB Domain Containing Protein 1 (CDCP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CUB Domain Containing Protein 1 (CDCP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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