FAS Chemi-Luminescent ELISA Kit (Mouse)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Fas

2°C to 8°C|-20°C

Tumor necrosis factor receptor superfamily member 6

Component: Amount
Fas Microplate: 96 Wells (12 x 8 Well strips)
Fas Lyophilized Standard: 2
100X Fas Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis (FAS). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Factor Related Apoptosis (FAS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Factor Related Apoptosis (FAS) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Factor Related Apoptosis (FAS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%