HTRA1 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

HtrA serine peptidase 1

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

NP_002766

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 78-95% - 96-104% - 90-98% - 85-103%
EDTA Plasma (n=5) - 98-105% - 79-101% - 82-94% - 78-102%
Heparin Plasma (n=5) - 83-91% - 92-99% - 82-91% - 83-97%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 82-103 - 90
EDTA Plasma (n=5) - 78-101 - 96
Heparin Plasma (n=5) - 98-105 - 101

Serum, plasma and other biological fluids