IL11 Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

interleukin 11

Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed:22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed:10026196, PubMed:23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed:23948300, PubMed:22253262).

NP_032376

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 92-105% - 78-88% - 89-98% - 91-99%
EDTA Plasma (n=5) - 94-101% - 86-94% - 83-98% - 88-95%
Heparin Plasma (n=5) - 82-98% - 81-105% - 96-103% - 80-90%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 80-96 - 88
EDTA Plasma (n=5) - 96-104 - 99
Heparin Plasma (n=5) - 98-105 - 101

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids