IL1RAP Chemi-Luminescent ELISA Kit (Mouse)

ELISA Kit

Wet Ice

interleukin 1 receptor accessory protein

Coreceptor for IL1RL2 in the IL-36 signaling system. Coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Recruits TOLLIP to the signaling complex. Does not bind to interleukin-1 alone; binding of IL1RN to IL1R1, prevents its association with IL1R1 to form a signaling complex. The cellular response is modulated through a non-signaling association with the membrane IL1R2 decoy receptor. Secreted forms (isoforms 2 and 3) associate with secreted ligand-bound IL1R2 and increase the affinity of secreted IL1R2 for IL1B; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Coreceptor for IL1RL1 in the IL-33 signaling system. Can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to PTPRD (PubMed:25908590). May play a role in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).

NP_001152790

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 95-103% - 96-104% - 78-91% - 80-90%
EDTA Plasma (n=5) - 80-99% - 92-101% - 98-105% - 85-92%
Heparin Plasma (n=5) - 87-97% - 97-104% - 80-101% - 84-91%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 89-102 - 98
EDTA Plasma (n=5) - 78-102 - 89
Heparin Plasma (n=5) - 79-88 - 83

Serum, plasma and other biological fluids