LEFTY1 Chemi-Luminescent ELISA Kit (Mouse)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Lefty1

2°C to 8°C|-20°C

NM_010094

Left-right determination factor 1

Component: Amount
Lefty1 Microplate: 96 Wells (12 x 8 Well strips)
Lefty1 Lyophilized Standard: 2
100X Lefty1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Left/Right Determination Factor 1 (LEFTY1) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Left/Right Determination Factor 1 (LEFTY1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%