CCL22 Chemi-Luminescent ELISA Kit (Mouse)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

Ccl22

2°C to 8°C|-20°C

NM_009137

C-C motif chemokine 22

Component: Amount
Ccl22 Microplate: 96 Wells (12 x 8 Well strips)
Ccl22 Lyophilized Standard: 2
100X Ccl22 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Macrophage Derived Chemokine (MDC). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Macrophage Derived Chemokine (MDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Macrophage Derived Chemokine (MDC) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Macrophage Derived Chemokine (MDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%