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MUC5B Chemi-Luminescent ELISA Kit (Human)

MUC5B Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD03813
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.0549 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Cervical mucin;cervical mucin MUC5B;high molecular weight salivary mucin MG1;MG1;MUC5;MUC-5B;MUC9;mucin 5,subtype B,tracheobronchial;Mucin-5 subtype B,tracheobronchial;mucin-5B;sublingual gland mucin.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	MUC5B
Gene Fullname	mucin 5B, oligomeric mucus/gel-forming
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Nucleotide accession_num	NM_002458
Protein accession_num	NP_002449
Protein name	Mucin-5B
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount MUC5B Microplate: 96 Wells (12 x 8 Well strips) MUC5B Lyophilized Standard: 2 100X MUC5B Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B (MUC5B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype B (MUC5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Mucin 5 Subtype B (MUC5B) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mucin 5 Subtype B (MUC5B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD03813.pdf

OKCD03813-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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