SPP1 Chemi-Luminescent ELISA Kit (Human)

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SPP1

2°C to 8°C|-20°C

NM_000582

Osteopontin

Component: Amount
SPP1 Microplate: 96 Wells (12 x 8 Well strips)
SPP1 Lyophilized Standard: 2
100X SPP1 Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteopontin (OPN) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%