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CCL5 Chemi-Luminescent ELISA Kit (Human)

CCL5 Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD03898
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology By Cell Type Carcinoma Hematology Leukemia Lymphoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	27.4 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	beta-chemokine RANTES;C-C motif chemokine 5;chemokine (C-C motif) ligand 5;D17S136E;eoCP;eosinophil chemotactic cytokine;RANTES;regulated upon activation,normally T-expressed,and presumably secreted;SCYA5;SISd;SIS-delta;small inducible cytokine subfamily A (Cys-Cys),member 5;Small-inducible cytokine A5;T cell-specific protein P228;T-cell specific protein p288;T-cell-specific protein RANTES;TCP228.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CCL5
Gene Fullname	C-C motif chemokine ligand 5
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils (PubMed:16791620, PubMed:1380064, PubMed:8525373, PubMed:9516414, PubMed:15923218). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Nucleotide accession_num	NM_002985
Protein accession_num	NP_002976
Protein name	C-C motif chemokine 5
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount CCL5 Microplate: 96 Wells (12 x 8 Well strips) CCL5 Lyophilized Standard: 2 100X CCL5 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 79-90% - 88-98% - 80-99% - 78-102% EDTA Plasma (n=5) - 86-101% - 89-96% - 89-103% - 88-101% Heparin Plasma (n=5) - 80-92% - 98-105% - 94-101% - 79-99%
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) level in the sample or standard.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 85-104 - 88 EDTA Plasma (n=5) - 96-103 - 99 Heparin Plasma (n=5) - 91-101 - 95
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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