TFPI Chemi-Luminescent ELISA Kit (Mouse)

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Tfpi

2°C to 8°C|-20°C

NM_011576

Tissue factor pathway inhibitor

Component: Amount
Tfpi Microplate: 96 Wells (12 x 8 Well strips)
Tfpi Lyophilized Standard: 2
100X Tfpi Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Tissue Factor Pathway Inhibitor (TFPI). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Tissue Factor Pathway Inhibitor (TFPI) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tissue Factor Pathway Inhibitor (TFPI) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%