TP53 Chemi-Luminescent ELISA Kit (Human)

ELISA Kit

Wet Ice

tumor protein p53

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seem to have to effect on cell-cycle regulation. Implicated in Notch signaling cross-over. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492).

NP_000537

Assay Methodology: Quantitative Sandwich ELISA

Chemiluminescent

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 80-92% - 83-102% - 85-99% - 80-98%
EDTA Plasma (n=5) - 96-104% - 86-101% - 86-102% - 78-93%
Heparin Plasma (n=5) - 90-97% - 91-98% - 88-99% - 93-101%

Matrix - Recovery range (%) - Average(%)
Serum (n=5) - 90-104 - 93
EDTA Plasma (n=5) - 96-105 - 101
Heparin Plasma (n=5) - 90-102 - 94

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids