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UCHL1 Chemi-Luminescent ELISA Kit (Rat)

UCHL1 Chemi-Luminescent ELISA Kit (Rat)

Item no.	OKCD04010
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology By Cell Type ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.026 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	neuron cytoplasmic protein 9.5;PGP 9.5;PGP9.5;ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase);ubiquitin carboxyl-terminal hydrolase isozyme L1;ubiquitin carboxy-terminal hydrolase L1;ubiquitin thioesterase L1;UCH-L1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Uchl1
Gene Fullname	ubiquitin C-terminal hydrolase L1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity (By similarity).
Nucleotide accession_num	NM_017237
Protein accession_num	NP_058933
Protein name	Ubiquitin carboxyl-terminal hydrolase isozyme L1
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Uchl1 Microplate: 96 Wells (12 x 8 Well strips) Uchl1 Lyophilized Standard: 2 100X Uchl1 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 82-101% - 78-101% - 81-104% - 92-101% EDTA Plasma (n=5) - 86-101% - 85-95% - 89-102% - 86-101% Heparin Plasma (n=5) - 83-101% - 85-101% - 85-92% - 87-97%
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) level in the sample or standard.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 85-99 - 95 EDTA Plasma (n=5) - 87-96 - 92 Heparin Plasma (n=5) - 96-105 - 101
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD04010.pdf

OKCD04010-datasheet.pdf

OKCD04010-safety-datasheet.pdf