TNFRSF10B ELISA Kit (Rat)

ELISA Kit

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TNFRSF10B

Tumor necrosis factor receptor superfamily member 10b

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Death receptor 5 (DR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Death receptor 5 (DR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Death receptor 5 (DR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Death receptor 5 (DR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Death receptor 5 (DR5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Death receptor 5 (DR5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%