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OSX ELISA Kit (Mouse)

OSX ELISA Kit (Mouse)

Item no.	OKCD04227
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Metabolism Diabetes ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	0.064 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	6430578P22Rik;C22;O;ost;Osx;transcription factor Sp7;zinc finger protein osterix.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Sp7
Gene Fullname	Sp7 transcription factor 7
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Transcriptional activator essential for osteoblast differentiation. Binds to SP1 and EKLF consensus sequences and to other G/C-rich sequences.
Nucleotide accession_num	NM_001348205.1
Protein accession_num	NP_001335134.1
Protein name	Transcription factor Sp7
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount Osx Microplate: 96 Wells (12 x 8 Well strips) Osx Lyophilized Standard: 2 100X Osx HRP-Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL TMB Substrate: 1 x 9 mL Stop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osterix (OSX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osterix (OSX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osterix (OSX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Osterix (OSX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osterix (OSX) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osterix (OSX) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD04227.pdf

OKCD04227-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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