IGK ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

gasdermin D

Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4, 5)-bisphosphate, as well as phosphatidylinositol (3, 4, 5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 83-93% - 90-104% - 89-98% - 94-102%
EDTA Plasma (n=5) - 86-93% - 78-89% - 87-97% - 87-105%
Heparin Plasma (n=5) - 96-104% - 94-104% - 93-101% - 84-102%

Matrix - Recovery range (%) - Average (%)
Serum (n=5) - 87-101 - 91
EDTA Plasma (n=5) - 91-105 - 95
Heparin Plasma (n=5) - 96-105 - 102

Serum, plasma and other biological fluids