FAM132A ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

C1q and TNF related 12

Insulin-sensitizing adipocyte-secreted protein (adipokine) that regulates glucose metabolism in liver and adipose tissue. Promotes glucose uptake in adipocytes and suppresses de novo glucose production in hepatocytes via the PI3K-Akt signaling pathway. Administration lead to reduction of blood glucose. Able to attenuate inflammation in fat tissue.

NP_001014980.1

Assay Methodology: Quantitative Sandwich ELISA

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 132, Member A (FAM132A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 132, Member A (FAM132A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 132, Member A (FAM132A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Family With Sequence Similarity 132, Member A (FAM132A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Tissue homogenates, cell lysates and other biological fluids