Comparison

FBLN3 ELISA Kit (Mouse)

Item no. OKCD04505
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Mouse (Murine, Mus musculus)
Sensitivity 6.0 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias EGF-containing fibulin-like extracellular matrix protein 1;FIBL-3;fibulin-3.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Efemp1
Gene Fullname
epidermal growth factor-containing fibulin-like extracellular matrix protein 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth (By similarity).
Nucleotide accession_num
NM_146015.2
Protein accession_num
NP_666127.2
Protein name
EGF-containing fibulin-like extracellular matrix protein 1
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit Components
Component: Amount
Mfap4 Microplate: 96 Wells (12 x 8 Well strips)
Mfap4 Lyophilized Standard: 2
100X Mfap4 HRP-Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL
Kit detection
Colorimetric
Kit duration
3h
Kit linearity
Sample - 1:2 - 1:4 - 1:8 - 1:16
Serum (n=5) - 99-105% - 86-104% - 78-94% - 91-99%
EDTA Plasma (n=5) - 91-98% - 78-94% - 87-94% - 90-98%
Heparin Plasma (n=5) - 92-99% - 98-105% - 89-96% - 96-103%
Kit principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit recovery
Matrix - Recovery range (%) - Average (%)
Serum (n=5) - 90-99 - 94
EDTA Plasma (n=5) - 87-94 - 90
Heparin Plasma (n=5) - 78-97 - 86
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibulin 3 (FBLN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, urine and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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