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CNTF Chemi-Luminescent ELISA Kit (Pig)

CNTF Chemi-Luminescent ELISA Kit (Pig)

Item no.	OKCD05505
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Molecular Targets for Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Pig (Porcine, Sus scrofa domesticus), Wild Boar
Sensitivity	< 3.2pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	HCNTF
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CNTF
Gene Fullname	ciliary neurotrophic factor
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy (By similarity).
Protein name	Ciliary neurotrophic factor
Kit Components	Component: Amount CNTF Microplate: 96 Wells (12 x 8 Well strips)CNTF Lyophilized Standard: 2100X CNTF HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Ciliary Neurotrophic Factor (CNTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ciliary Neurotrophic Factor (CNTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ciliary Neurotrophic Factor (CNTF) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ciliary Neurotrophic Factor (CNTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD05505.pdf

OKCD05505-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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