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HGF Chemi-Luminescent ELISA Kit (Rabbit)

HGF Chemi-Luminescent ELISA Kit (Rabbit)

Item no.	OKCD05519
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Adenocarcinoma Mesothelioma ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Rabbit (Oryctolagus cuniculus)
Sensitivity	< 0.58pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	hepatocyte growth factor;hepatocyte growth factor (hepapoietin A; scatter factor);Hepatopoietin-A;Scatter factor.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	HGF
Gene Fullname	hepatocyte growth factor
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization.
Nucleotide accession_num	NM_001168707.1
Protein accession_num	NP_001162178.1
Protein name	Hepatocyte growth factor
Kit Components	Component: Amount HGF Microplate: 96 Wells (12 x 8 Well strips)HGF Lyophilized Standard: 2100X HGF HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Hepatocyte Growth Factor (HGF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hepatocyte Growth Factor (HGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hepatocyte Growth Factor (HGF) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD05519.pdf

OKCD05519-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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