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CCL14 Chemi-Luminescent ELISA Kit (Human)

CCL14 Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD05561
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation By Cell Type Carcinoma Hematology Leukemia ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Heart & Blood Cancer CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.54pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	C-C motif chemokine 14;CC-1;CC-3;chemokine (C-C motif) ligand 14;chemokine CC-1/CC-3;chemokine CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;hemofiltrate CC chemokine 1;MCIF;NCC2;NCC-2;new CC chemokine 2;SCYA14;SCYL2;small inducible cytokine subfamily A (Cys-Cys),member 14;small-inducible cytokine A14;SY14.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CCL14
Gene Fullname	C-C motif chemokine ligand 14
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1, 000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
Nucleotide accession_num	NM_032962.4
Protein accession_num	NP_116738.1
Protein name	C-C motif chemokine 14
Kit Components	Component: Amount CCL14 Microplate: 96 Wells (12 x 8 Well strips)CCL14 Lyophilized Standard: 2100X CCL14 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Ligand 14 (CCL14). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Ligand 14 (CCL14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-C-Motif Ligand 14 (CCL14) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 14 (CCL14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Ligand 14 (CCL14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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