Comparison

HYAL1 Chemi-Luminescent ELISA Kit (Human)

Item no. OKCD05563
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Human (Homo sapiens)
Sensitivity < 0.28ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias HYAL-1;hyaluronidase-1;hyaluronoglucosaminidase 1;Hyaluronoglucosaminidase-1;luCa-1;LUCA1;lung carcinoma protein 1;MPS9;NAT6;plasma hyaluronidase;tumor suppressor LUCA-1.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
HYAL1
Gene Fullname
hyaluronidase 1
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
May have a role in promoting tumor progression. May block the TGFB1-enhanced cell growth.
Nucleotide accession_num
NM_033159.3
Protein accession_num
NP_149349.2
Protein name
Hyaluronidase-1
Kit Components
Component: Amount
HYAL1 Microplate: 96 Wells (12 x 8 Well strips)HYAL1 Lyophilized Standard: 2100X HYAL1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase (HAase). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Hyaluronidase (HAase). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Hyaluronidase (HAase) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hyaluronidase (HAase) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hyaluronidase (HAase) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma, tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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