Comparison

CALR Chemi-Luminescent ELISA Kit (Rat)

Item no. OKCD05568
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Rat (Rattus norvegicus)
Sensitivity < 1.21pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CABP3;CALBP;calcium-binding protein 3;calregulin;calreticulin;CRP55;endoplasmic reticulum resident protein 60;ERp60;HACBP.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Calr
Gene Fullname
calreticulin
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.
Nucleotide accession_num
NM_022399.2
Protein accession_num
NP_071794.1
Protein name
Calreticulin
Kit Components
Component: Amount
CALR Microplate: 96 Wells (12 x 8 Well strips)CALR Lyophilized Standard: 2100X CALR HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 2 mLSubstrate Diluent: 1 x 20 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Calreticulin (CALR). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Calreticulin (CALR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Calreticulin (CALR) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calreticulin (CALR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calreticulin (CALR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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