Comparison

IFNA1 Chemi-Luminescent ELISA Kit (Rat)

Item no. OKCD05589
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications CLIA, IA
Specific against Rat (Rattus norvegicus)
Sensitivity < 0.51pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Ifna1;Ifna1l1;IFN-alpha1;interferon alpha-1;interferon-alpha 1;interferon-alpha 1-like 1.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
Ifna5
Gene Fullname
interferon, alpha 5
Reconstitution and storage
2°C to 8°C|-20°C
Description of target
Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
Nucleotide accession_num
NM_001014786.1
Protein accession_num
NP_001014786.1
Protein name
Interferon alpha-1
Kit Components
Component: Amount
IFNA1 Microplate: 96 Wells (12 x 8 Well strips)IFNA1 Lyophilized Standard: 2100X IFNA1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mL100X Luminol Substrate: 1 x 9 mLSubstrate Diluent: 1 x 6 mL
Kit detection
Chemiluminescent
Kit duration
2h, 40min
Kit principle
The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha 13 (IFNa13). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha 13 (IFNa13). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha 13 (IFNa13) level in the sample or standard.
Kit reproducibility
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha 13 (IFNa13) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha 13 (IFNa13) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type
Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close