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CD44 ELISA Kit (Rat)

CD44 ELISA Kit (Rat)

Item no.	OKCD06646
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Cell Biology Cell Movement Cell Adhesion ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	< 0.65ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	CD44 antigen;CD44A;Cell surface glycoprotein CD44 (hyaluronate binding protein);ECMR-III;extracellular matrix receptor III;GP90 lymphocyte homing/adhesion receptor;hermes antigen;HUTCH-I;hyaluronate receptor;METAA;PGP-1;PGP-I;phagocytic glycoprotein 1;phagocytic glycoprotein I;RHAMM.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Cd44
Gene Fullname	CD44 molecule (Indian blood group)
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. In cancer cells, may play an important role in invadopodia formation. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Receptor for LGALS9; the interaction enhances binding of SMAD3 to the FOXP3 promoter, leading to up-regulation of FOXP3 expression and increased induced regulatory T (iTreg) cell stability and suppressive function.
Nucleotide accession_num	XP_006234693.1
Protein accession_num	XM_006234631.3
Protein name	CD44 antigen
Kit Components	Component: Amount CD44 Microplate: 96 Wells (12 x 8 Well strips)CD44 Lyophilized Standard: 2100X CD44 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Homing Associated Cell Adhesion Molecule (HCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Homing Associated Cell Adhesion Molecule (HCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Homing Associated Cell Adhesion Molecule (HCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Homing Associated Cell Adhesion Molecule (HCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD06646.pdf

OKCD06646-datasheet.pdf

OKCD06646-safety-datasheet.pdf