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FOXO3 ELISA Kit (Mouse)

FOXO3 ELISA Kit (Mouse)

Item no.	OKCD06778
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	< 0.054ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Fox,FKHR,Fkhr2,C76856,FKHRL1,Foxo3a,1110048B16Rik,2010203A17Rik
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	FOXO3
Gene Fullname	forkhead box O3
Reconstitution and storage	2°C to 8°C, -20°C
Description of target	Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. recognizes and binds to the dna sequence 5'-.taaa.a-3'. participates in post-transcriptional regulation of myc: following phosphorylation by mapkapk5, promotes induction of mir-34b and mir-34c expression, 2 post-transcriptional regulators of myc that bind to the 3'utr of myc transcript and prevent its translation.
Nucleotide accession_num	NM_019740.2
Protein accession_num	NP_062714.1
Protein name	forkhead box protein O3
Kit Components	Component: Amount FOXO3 Microplate: 96 Wells (12 x 8 Well strips)FOXO3 Lyophilized Standard: 2100X FOXO3 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colormetric\|Regular detection
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Forkhead Box Protein O3 (FOXO3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Forkhead Box Protein O3 (FOXO3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Forkhead Box Protein O3 (FOXO3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Forkhead Box Protein O3 (FOXO3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Forkhead Box Protein O3 (FOXO3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Forkhead Box Protein O3 (FOXO3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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