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C3 ELISA Kit (Bovine)

C3 ELISA Kit (Bovine)

Item no.	OKCD06918
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neuroinflammation Aging & Neurological Disorders ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	< 1.28ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	complement C3;complement component 3.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	C3
Gene Fullname	complement C3
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates (By similarity).
Nucleotide accession_num	NM_001040469.2
Protein accession_num	NP_001035559.2
Protein name	Complement C3
Kit Components	Component: Amount C3 Microplate: 96 Wells (12 x 8 Well strips)C3 Lyophilized Standard: 2100X C3 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Complement Component 3 (C3) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Complement Component 3 (C3) and unlabeled Complement Component 3 (C3) (Standards or samples) with the pre-coated antibody specific to Complement Component 3 (C3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Complement Component 3 (C3) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Complement Component 3 (C3) in the sample.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 3 (C3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 3 (C3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD06918.pdf

OKCD06918-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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