GAPDH ELISA Kit (Mouse)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

glyceraldehyde-3-phosphate dehydrogenase|glyceraldehyde-3-phosphate dehydrogenase, pseudogene 15

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

NP_001276655.1

Component: Amount
GAPDH Microplate: 96 Wells (12 x 8 Well strips)GAPDH Lyophilized Standard: 2100X GAPDH HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL

3h

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%