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GDF5 ELISA Kit (Human)

GDF5 ELISA Kit (Human)

Item no.	OKCD07997
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Molecular Targets for Neuroscience Targets for Rodent Neurosciences Neural Signaling By Cell Type Carcinoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis Adenocarcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Colorectal Cancer Immunostaining Neuron Nuclear Markers
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 6.7pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	BDA1C;BMP14;BMP-14;bone morphogenetic protein 14;Cartilage-derived morphogenetic protein 1;cartilage-derived morphogenetic protein-1;CDMP1;DUPANS;growth/differentiation factor 5;LAP4;LAP-4;lipopolysaccharide-associated protein 4;LPS-associated protein 4;OS5;radotermin;SYM1B;SYNS2.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	GDF5
Gene Fullname	growth differentiation factor 5
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Growth factor involved in bone and cartilage formation. During cartilage development regulates differentiation of chondrogenic tissue through two pathways. Firstly, positively regulates differentiation of chondrogenic tissue through its binding of high affinity with BMPR1B and of less affinity with BMPR1A, leading to induction of SMAD1-SMAD5-SMAD8 complex phosphorylation and then SMAD protein signaling transduction (PubMed:24098149, PubMed:21976273, PubMed:15530414, PubMed:25092592). Secondly, negatively regulates chondrogenic differentiation through its interaction with NOG (PubMed:21976273). Required to prevent excessive muscle loss upon denervation. This function requires SMAD4 and is mediated by phosphorylated SMAD1/5/8 (By similarity). Binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes (PubMed:11276205).
Nucleotide accession_num	NM_000557.4
Protein accession_num	NP_000548.2
Protein name	Growth/differentiation factor 5
Kit Components	Component: Amount GDF5 Microplate: 96 Wells (12 x 8 Well strips)GDF5 Lyophilized Standard: 2100X GDF5 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 5 (GDF5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 5 (GDF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 5 (GDF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Growth Differentiation Factor 5 (GDF5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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