TNFSF15 ELISA Kit (Human)

ELISA Kit

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TNFSF15

2°C to 8°C|-20°C

NM_001204344.1

Tumor necrosis factor ligand superfamily member 15

Colorimetric

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Like Ligand 1A (TL1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Like Ligand 1A (TL1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Like Ligand 1A (TL1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNF Like Ligand 1A (TL1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Serum, plasma, tissue homogenates and other biological fluids