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SCG5 ELISA Kit (Human)

SCG5 ELISA Kit (Human)

Item no.	OKCD08265
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Metabolism Diabetes Glucose Homeostasis Regulation of Appetite ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 3.5pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	7B2;neuroendocrine protein 7B2;P7B2;pituitary polypeptide;prohormone convertase chaperone;Secretogranin V;secretogranin-5;secretory granule endocrine protein I;secretory granule,neuroendocrine protein 1 (7B2 protein);SGNE1;SgV.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SCG5
Gene Fullname	secretogranin V
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.
Nucleotide accession_num	NM_001144757.2
Protein accession_num	NP_001138229.1
Protein name	Neuroendocrine protein 7B2
Kit Components	Component: Amount SCG5 Microplate: 96 Wells (12 x 8 Well strips)SCG5 Lyophilized Standard: 2100X SCG5 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretogranin V (SCG5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Secretogranin V (SCG5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Secretogranin V (SCG5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Secretogranin V (SCG5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Secretogranin V (SCG5) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Secretogranin V (SCG5) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD08265.pdf

OKCD08265-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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