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TWIST1 ELISA Kit (Human)

TWIST1 ELISA Kit (Human)

Item no.	OKCD08266
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience Targets for Rodent Neurosciences Aging & Neurological Disorders Neural Signaling Neural Epigenetics Neural Development Cell Biology Cell Movement Cell Adhesion Cell Culture Cytokines ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Immunostaining Stem Cell Markers
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.063ng/mL
Citations	The association between HPV gene expression, inflammatory agents and cellular genes involved in EMT in lung cancer tissue. BMC Cancer. 20, 916 (2020). 32972386
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ACS3;B-HLH DNA binding protein;bHLHa38;BPES2;BPES3;class A basic helix-loop-helix protein 38;CRS;CRS1;CSO;H-twist;SCS;SWCOS;TWIST;twist basic helix-loop-helix transcription factor 1;twist homolog 1;TWIST homolog of drosophila;twist-related protein 1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	TWIST1
Gene Fullname	twist family bHLH transcription factor 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).
Nucleotide accession_num	NM_000474.3
Protein accession_num	NP_000465.1
Protein name	Twist-related protein 1
Kit Components	Component: Amount TWIST1 Microplate: 96 Wells (12 x 8 Well strips)TWIST1 Lyophilized Standard: 2100X TWIST1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Twist Transcription Factor (TWIST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Twist Transcription Factor (TWIST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Twist Transcription Factor (TWIST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Twist Transcription Factor (TWIST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Twist Transcription Factor (TWIST) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD08266.pdf

OKCD08266-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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