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TRAF6 ELISA Kit (Human)

TRAF6 ELISA Kit (Human)

Item no.	OKCD08976
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neural Lineage Markers Neuroinflammation Metabolism Diabetes Aging & Neurological Disorders By Cell Type Blastoma Carcinoma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Obesity Cell Culture Cytokines Cell Status Necrosis Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Breast Cancer Immunostaining Neurodegeneration Markers
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.053ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	E3 ubiquitin-protein ligase TRAF6;interleukin-1 signal transducer;MGC:3310;RING finger protein 85;RING-type E3 ubiquitin transferase TRAF6;RNF85;TNF receptor-associated factor 6;TNF receptor-associated factor 6,E3 ubiquitin protein ligase.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	TRAF6
Gene Fullname	TNF receptor associated factor 6
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation. Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
Nucleotide accession_num	NM_004620.3
Protein accession_num	NP_004611.1
Protein name	TNF receptor-associated factor 6
Kit Components	Component: Amount TRAF6 Microplate: 96 Wells (12 x 8 Well strips)TRAF6 Lyophilized Standard: 2100X TRAF6 HRP-Detector Anitbody: 1 x 120 Ã‚uL100X Avidin-HRP Conjugate: 1 x 120 Ã‚uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TNF Receptor Associated Factor 6 (TRAF6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF Receptor Associated Factor 6 (TRAF6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TNF Receptor Associated Factor 6 (TRAF6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF Receptor Associated Factor 6 (TRAF6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD08976.pdf

OKCD08976-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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