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PPARGC1A ELISA Kit (Rat)

PPARGC1A ELISA Kit (Rat)

Item no.	OKCD09047
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Metabolism Diabetes Glucose Homeostasis Cholesterol & Lipid Metabolism Molecular Targets for Neuroscience By Cell Type Carcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay By Organ Thyroid Cancer Immunostaining Other Lipid\Cholesterol Metabolism Modulators
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	< 0.125ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	LRPGC1;peroxisome proliferative activated receptor gamma coactivator 1;peroxisome proliferative activated receptor,gamma,coactivator 1 alpha;peroxisome proliferator-activated receptor gamma coactivator 1-alpha;PGC-1alpha;PGC-1-alpha;PGC-1v;PGCvf;PGCvf1;PGCvf-1;PPAR gamma coactivator 1alpha variant form-1;PPAR-gamma coactivator 1-alpha;Ppargc1;PPARGC-1-alpha;Ppargc1a-vf1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	Ppargc1a
Gene Fullname	PPARG coactivator 1 alpha
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK (By similarity).
Nucleotide accession_num	NM_031347.1
Protein accession_num	NP_112637.1
Protein name	Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Kit Components	Component: Amount PPARGC1A Microplate: 96 Wells (12 x 8 Well strips)PPARGC1A Lyophilized Standard: 2100X PPARGC1A HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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