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HTRA1 ELISA Kit (Human)

HTRA1 ELISA Kit (Human)

Item no.	OKCD09313
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Aging & Neurological Disorders By Cell Type Carcinoma Hematology Lymphoma Myeloma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Astrocytoma Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 13.2pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ARMD7;CADASIL2;CARASIL;high-temperature requirement A serine peptidase 1;HtrA;IGFBP5-protease;L56;ORF480;protease,serine,11 (IGF binding);PRSS11;Serine protease 11;serine protease HTRA1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	HTRA1
Gene Fullname	HtrA serine peptidase 1
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
Nucleotide accession_num	NM_002775.4
Protein accession_num	NP_002766.1
Protein name	Serine protease HTRA1
Kit Components	Component: Amount HTRA1 Microplate: 96 Wells (12 x 8 Well strips)HTRA1 Lyophilized Standard: 2100X HTRA1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 9 mLStop Solution: 1 x 6 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Temperature Requirement Factor A1 (HTRA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Temperature Requirement Factor A1 (HTRA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Temperature Requirement Factor A1 (HTRA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Temperature Requirement Factor A1 (HTRA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Temperature Requirement Factor A1 (HTRA1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Temperature Requirement Factor A1 (HTRA1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD09313.pdf

OKCD09313-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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