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SIVA1 ELISA Kit (Human)

SIVA1 ELISA Kit (Human)

Item no.	OKCD09360
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Oncology By Cell Type Carcinoma Hematology Leukemia Lymphoma Melanoma Cell Biology Cell Status Apoptosis ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.068ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	apoptosis regulatory protein Siva;CD27-binding (Siva) protein;CD27-binding protein;CD27BP;SIVA;Siva-1;Siva-2.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	SIVA1
Gene Fullname	SIVA1 apoptosis inducing factor
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Induces CD27-mediated apoptosis. Inhibits BCL2L1 isoform Bcl-x(L) anti-apoptotic activity. Inhibits activation of NF-kappa-B and promotes T-cell receptor-mediated apoptosis.
Nucleotide accession_num	NM_006427.3
Protein accession_num	NP_006418.2
Protein name	Apoptosis regulatory protein Siva
Kit Components	Component: Amount SIVA1 Microplate: 96 Wells (12 x 8 Well strips)SIVA1 Lyophilized Standard: 2100X SIVA1 HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD27 Binding Protein (CD27BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD27 Binding Protein (CD27BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD27 Binding Protein (CD27BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CD27 Binding Protein (CD27BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CD27 Binding Protein (CD27BP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CD27 Binding Protein (CD27BP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD09360.pdf

OKCD09360-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/25/2025

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