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HIST1H4B MORE ELISA Kit (Rat)

HIST1H4B MORE ELISA Kit (Rat)

Item no.	OKCD09442
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neural Epigenetics ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Rat (Rattus norvegicus)
Sensitivity	< 0.069ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	germinal histone H4;H1ft;H4c2;H4f16;Hist1h4b;Hist4;Hist4h4;histone 1,H2ao;histone 1,H4m;histone 2,H4;histone cluster 1,H4b;histone cluster 2,H4;histone H2A type 1-E;histone H4;histone H4-like;RGD1565073.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	H4c3\|Hist1h2ao\|Hist1h4b\|Hist1h4m\|Hist2h4\|Hist2h4a\|LOC100912564\|RGD1562378
Gene Fullname	H4 clustered histone 3\|histone cluster 1 H4 family member B\|histone cluster 1, H2ao\|histone cluster 1, H4m\|histone cluster 2 H4 family member A\|histone cluster 2, H4\|histone H4 variant H4-v.1\|histone H4-like
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Nucleotide accession_num	NM_001123469.1
Protein accession_num	NP_001116941.1
Protein name	Histone H4
Kit Components	Component: Amount HIST1H4B Microplate: 96 Wells (12 x 8 Well strips)HIST1H4B Lyophilized Standard: 2100X HIST1H4B HRP-Detector Anitbody: 1 x 120 uL100X Avidin-HRP Conjugate: 1 x 120 uLStandard Diluent: 1 x 20 mLDetector Antibody Diluent: 1 x 12 mLConjugate Diluent: 1 x 12 mL30X Wash Buffer: 1 x 20 mLTMB Substrate: 1 x 2 mLStop Solution: 1 x 20 mL
Kit detection	Colorimetric
Kit duration	3h
Kit principle	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone Cluster 1, H4a (HIST1H4A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histone Cluster 1, H4a (HIST1H4A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histone Cluster 1, H4a (HIST1H4A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Histone Cluster 1, H4a (HIST1H4A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Histone Cluster 1, H4a (HIST1H4A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Histone Cluster 1, H4a (HIST1H4A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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