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BAG3 ELISA Kit (Human)

BAG3 ELISA Kit (Human)

Item no.	OKDD00144
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology Neuroscience Neuroinflammation Protein Aggregation, Clearance & Misfolding By Cell Type Carcinoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.069 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	BAG family molecular chaperone regulator 3;BAG-3;BCL2 associated athanogene 3;Bcl-2-associated athanogene 3;BCL2-binding athanogene 3;bcl-2-binding protein Bis;Bcl-2-binding protein BIS transcript 2;BIS;CAIR-1;docking protein CAIR-1;MFM6.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	62 kDa
Gene symbol	BAG3
Gene Fullname	BAG cochaperone 3
Protein size	575
Reconstitution and storage	Store as indicated in product manual.
Description of target	Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
Nucleotide accession_num	NM_004281.3
Protein accession_num	NP_004272.2
Protein name	BAG family molecular chaperone regulator 3
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of BAG3.No significant cross-reactivity or interference between BAG3 and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology BAG3 ELISA Kit (Human) (OKDD00144) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for BAG3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAG3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BAG3 captured in well.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAG3 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAG3 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, Plasma, Tissue homogenates, Cell lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKDD00144.pdf

OKDD00144-datasheet.pdf

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Amount: 96 tests

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Delivery expected until 12/25/2025

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