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IRF9 ELISA Kit (Human)

IRF9 ELISA Kit (Human)

Item no.	OKDD00345
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology Neuroscience Targets for Rodent Neurosciences By Cell Type Carcinoma Hematology Leukemia Lymphoma Melanoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	< 0.042 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	IFN-alpha-responsive transcription factor subunit;interferon regulatory factor 9;interferon-stimulated gene factor 3 gamma;interferon-stimulated transcription factor 3,gamma (48kD);interferon-stimulated transcription factor 3,gamma 48kDa;IRF-9;ISGF3;ISGF-3 gamma;ISGF3 p48 subunit;ISGF3G;p48;transcriptional regulator ISGF3 subunit gamma.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	44 kDa
Gene symbol	IRF9
Gene Fullname	interferon regulatory factor 9
Protein size	393
Reconstitution and storage	Store as indicated in product manual.
Description of target	Transcription factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Nucleotide accession_num	NM_006084.4
Protein accession_num	NP_006075.3
Protein name	Interferon regulatory factor 9
Manufacturer - Specificity	This assay has high sensitivity and excellent specificity for detection of IRF9.No significant cross-reactivity or interference between IRF9 and analogues was observed.
Assay info	Quantitative Sandwich ELISA
Kit duration	~4 Hours
Kit principle	Aviva Systems Biology IRF9 ELISA Kit (Human) (OKDD00345) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for IRF9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRF9 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IRF9 captured in well.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IRF9 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IRF9 were tested on 3 different plates, 8 replicates in each plate. Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, Plasma, Tissue homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKDD00345.pdf

OKDD00345-datasheet.pdf

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Amount: 96 tests

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Delivery expected until 12/25/2025

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