TMEM2L ELISA Kit (Human)

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153 kDa

cell migration inducing hyaluronidase 1

Store as indicated in product manual.

NM_001293298.1

Cell migration-inducing and hyaluronan-binding protein

Quantitative Sandwich ELISA

Aviva Systems Biology TMEM2L ELISA Kit (Human) (OKDD00566) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for TMEM2L has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TMEM2L is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample TMEM2L captured in well.

Tissue homogenates, Cell lysates and Other biological fluids