MCP1 ELISA Kit (Monkey)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

11 kDa

C-C motif chemokine ligand 2

Store as indicated in product manual.

NM_001284872.1

C-C motif chemokine 2

Quantitative Sandwich ELISA

Aviva Systems Biology MCP1 ELISA Kit (Monkey) (OKDD00615) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for MCP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MCP1 captured in well.

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates.