YAP1 ELISA Kit (Mouse)

ELISA Kit

Wet Ice

Yap1

448

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction.

NP_001164618.1

This assay has high sensitivity and excellent specificity for detection of YAP1.No significant cross-reactivity or interference between YAP1 and analogues was observed.

~4 Hours

Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

Mean Intra-Assay: CV<10%

Mean Inter-Assay: CV<12%