C-Peptide ELISA Kit (Dog)

Enzyme-linked Immunosorbent assay-Competitive

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Store as indicated in product manual.

C-Peptide

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology C-Peptide ELISA Kit (Dog) (OKEH00455) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog C-Peptide antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Dog C-Peptide and incubated. The Dog C-Peptide found in the sample or standards competes with the biotinylated Dog C-Peptide for limited binding sites on the immobilized anti-Dog C-Peptide antibody. Excess unbound biotinylated Dog C-Peptide and sample or standard Dog C-Peptide is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Dog C-Peptide captured in the well and inversely proportional to the amount of Dog C-Peptide which was contained in the sample or standard.

Mean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 8.2% (n = 20)