Melatonin ELISA Kit

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Biochemicals Hormones

Store as indicated in product manual.

Melatonin

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Melatonin ELISA Kit (OKEH02566) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Melatonin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Melatonin and incubated. The Melatonin found in the sample or standards competes with the biotinylated Melatonin for limited binding sites on the immobilized anti-Melatonin antibody. Excess unbound biotinylated Melatonin and sample or standard Melatonin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Melatonin captured in the well and inversely proportional to the amount of Melatonin which was contained in the sample or standard.

Mean Intra-assay CV%: < 4.1% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)