Vitamin B6 ELISA Kit

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Biochemicals Hormones

Store as indicated in product manual.

Vitamin B6

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Vitamin B6 ELISA Kit (OKEH02570) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Vitamin B6 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Vitamin B6 and incubated. The Vitamin B6 found in the sample or standards competes with the biotinylated Vitamin B6 for limited binding sites on the immobilized anti-Vitamin B6 antibody. Excess unbound biotinylated Vitamin B6 and sample or standard Vitamin B6 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Vitamin B6 captured in the well and inversely proportional to the amount of Vitamin B6 which was contained in the sample or standard.

Serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids