Phosphatidylserine ELISA Kit

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/ELISA Kits/Biochemicals Hormones

Store as indicated in product manual.

Phosphatidylserine

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Phosphatidylserine ELISA Kit (OKEH02601) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Phosphatidylserine antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Phosphatidylserine and incubated. The Phosphatidylserine found in the sample or standards competes with the biotinylated Phosphatidylserine for limited binding sites on the immobilized anti-Phosphatidylserine antibody. Excess unbound biotinylated Phosphatidylserine and sample or standard Phosphatidylserine is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Phosphatidylserine captured in the well and inversely proportional to the amount of Phosphatidylserine which was contained in the sample or standard.

Mean Intra-assay CV%: < 4.5% (n = 20)
Mean Inter-assay CV%: < 8.4% (n = 20)